Related Experiment Video
Updated: Aug 18, 2026
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
Published on: September 15, 2016
Microarray-based genotyping for blood groups: comparison of gene array and 5'-nuclease assay techniques with human
Peter Bugert1, Simon McBride, Graham Smith
1Institute of Transfusion Medicine and Immunology, University of Heidelberg, Faculty of Clinical Medicine, Mannheim, Germany.
Background:
Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins.
Study Design And Methods:
By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems.
Results:
Unambiguous and concordant results were obtained with both techniques for all samples.
Conclusion:
The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.
