Related Experiment Video
Updated: Aug 3, 2026
Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method
Published on: May 19, 2023
[Content analysis of bilirubin in niuhuang compound suppository by HPLC]
Jing Chen1, Guoping Gan, Zai'an He
1Hubei College of Traditional Chinese Medicine, Wuhan 430061.
Objective:
To establish a method of determinating content of bilirubin in Niuhuang Compound Suppository by HPLC.
Method:
C18 column was used. The mobile phase was consisted of dimethylsulfoxide: acetonitrile: 0.5% acetic ammonium (1:1.4:1). Detector wavelength was 456 nm. The extraction solution for bilirubin was consisted of dlimethylsulfoxide: acetonitrile = 9:4.
Results:
The linearity was obtained over the range of 0.107 - 2.14 microg. The mean recovery was 101.0%, RSD = 0.518% (n = 5).
Conclusion:
The method was sensitive, simple and accurate.
More Related Videos
08:44Elucidation of the Material Basis of Yiqi Qingjie Formula Against IgA Nephropathy Using UHPLC-Q-Orbitrap HRMS Integrated with Network Pharmacology
Published on: May 19, 2026
07:45Technical Approach for Structural Analysis of an Unknown Compound in Huoxiang Zhengqi Oral Liquid based on Linear Ion Trap Mass Spectrometry
Published on: April 3, 2026