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Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
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The contested role of uracil DNA glycosylase in immunoglobulin gene diversification

Simonne Longerich1, Ursula Storb

  • 1Department of Molecular Genetics and Cell Biology, Committee on Immunology, University of Chicago, IL 60637, USA.

Trends in Genetics : TIG
|April 27, 2005
PubMed

Class switch recombination (CSR) and somatic hypermutation (SHM) of immunoglobulin (Ig) genes are initiated by the activation-induced cytosine deaminase AID. The resulting uracils in Ig genes were believed to be removed by the uracil glycosylase (UNG) and the resulting abasic sites treated in an error-prone fashion, creating breaks in the Ig switch regions and mutations in the variable regions. A recent report suggests that UNG does not act as a glycosylase in CSR and SHM but rather has unknown activity subsequent to DNA breaks that were created by other mechanisms.

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