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Updated: Oct 9, 2026

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
Published on: May 3, 2014
A complex relationship: pre-mRNA 3' processing and transcription termination
Agata Stępień1, Martyna Plens-Gałąska1, Joanna Kwiatkowska1
1Center for Advanced Technologies, Adam Mickiewicz University, 61-614 Poznań, Poland; Department of Molecular and Cellular Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznań, Poland.
Abstract:
Two molecular events occur at gene ends: 3'-end processing of pre-mRNA and transcription termination. Decades of studies have functionally coupled them, yet we discuss evidence that changes in these processes can occur in opposing directions. Acknowledging this paradox is necessary to gain a real understanding of how gene ends are shaped.
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