[Influence of hypoxia on leptin and leptin receptor gene expression of C57BL/6J mice]
Related Experiment Video
Updated: Aug 18, 2026
Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing
Published on: October 25, 2019
1Department of Gerontology, Third People's Hospital, Chongqing 400014, China.
Objective:
To investigate the influence of hypoxia on leptin and leptin receptor gene expression of C57BL/6J mice.
Methods:
C57BL/6J mice, exposed to hypoxia in the man-made auto pressure and hypoxia control cabin (XQ-I), were divided into 3 groups: the normal control group, 24 h hypoxia group and 48 h hypoxia group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of leptin and leptin receptor.
Results:
(1) Compared with the normal control group (0.508 +/- 0.207), the leptin mRNA level of 24 h hypoxia group (0.903 +/- 0.190) and 48 h hypoxia group (0.856 +/- 0.336) were up-regulated markedly (all < 0.05); (2) Compared with the normal control group (Ra 0.630, Rb 0.258 +/- 0.049, Rc 0.133), the Ra, Rb, Rc mRNA levels of 24 h hypoxia group (Ra 0.724, Rb 0.381 +/- 0.038, Rc 0.299) and 48 h hypoxia group (Ra 0.700, Rb 0.345 +/- 0.042, Rc 0.292) were up-regulated to different degrees.
Conclusions:
As an independent factor, hypoxia can stimulate increased expression of leptin, leptin receptor Ra, Rb, Rc mRNA. It is suggested that leptin play an important role in positive regulation of respiratory function.
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