[DNA macroarray analysis of gene expression changes in rat brain after single administration of 2-aminoadamantane...

We have used the Rat Atlas cDNA Array ("BD Bioscience") to assess changes in mRNA expression of 588 genes in rat brain after acute treatment of 2-aminoadamantane compound--Ladasten. Drug exhibits the psychostimulating and anxyolitic actions. The analysis of results of hybridization on macrochips and their corroboration by quantitative real-time RT-PCR has allowed to reveal 12 genes, expression of which changes in response to ladasten in rat brain cells. The GAT3 and CARBH genes should be considered as primary pharmacologically significant targets and the changes of their functional conditions allows to explain the distinct mechanisms of anxyolitic properties of the drug. It was shown that Ladasten induced genes are involved in the different signalling pathways (APC, Rb, PKCIP, PMCA), genes encoding the cytosceletal proteins (Tubal, actin), synaptic proteins (Syn IA&IB, PLP) and metabolism enzymes (Gapdh, NSE). It is possible to assume, that proteins, encoded by the given genes participate in the compensatory and/or neuroplastic adaptation to biochemical effects of Ladasten.