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Updated: Aug 18, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Published on: January 16, 2017
Cloning and expression of a novel isoform of heterogeneous nuclear ribonucleoprotein-R
Jia Huang1, Xian-hua Chen, Kun Wu
1Laboratory of Genomic Physiology and Center for Brain Research, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, People's Republic of China.
Abstract:
In the present study, we cloned a novel isoform of heterogeneous nuclear ribonucleoprotein-R (hnRNP-R), hnRNP-R2. Compared with the previously reported hnRNP-R (hnRNP-R1), it lacks the exon 5. Two proteins are both nuclear. The expression of hnRNP-R1 is not tissue-specific and is strikingly higher than that of hnRNR-R2. In comparison, the expression of hnRNP-R2 is low and neural-specific. The expression of two isoforms is differentially regulated during brain development. The results suggest that hnRNP-R may contribute to the regulation of neural development through the variation of hnRNP-R components in the hnRNP complex.
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