Related Experiment Video
Updated: Aug 18, 2026

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
Published on: July 28, 2022
Effect of bathing on skin flora of preterm newborns
Maria Luzia Chollopetz da Cunha1, Renato S Procianoy
1Department of Pediatrics, Newborn Section, Universidade Federal do Rio Grande do Sul and Hospital de Clínicas de Porto Alegre, RS, Brazil.
Insights
Bathing preterm newborns with water alone or with mild soap and water similarly impacts skin flora. Both methods effectively reduce bacterial colonies on neonate skin.
Area of Science:
- Neonatal care
- Microbiology
- Dermatology
Background:
- Preterm newborns have immature skin barriers, increasing infection risk.
- Skin colonization by microorganisms plays a crucial role in neonatal health and disease.
- Optimal bathing practices are essential for maintaining skin integrity and preventing infections in vulnerable infants.
Purpose of the Study:
- To compare the effects of water-only bathing versus mild soap and water bathing on the skin microflora of preterm newborns.
- To evaluate the impact of different bathing interventions on bacterial colonization in preterm infants.
Main Methods:
- A randomized blinded clinical trial involving 73 preterm newborns (gestational age 28–35 weeks, birth weight 800–1800 g).
- Infants were assigned to receive daily baths with either water only or mild soap and water for at least 7 days.
- Axillary bacterial cultures were collected before and 30 minutes after bathing on the final day.
Main Results:
- Coagulase-negative Staphylococcus was the most common microorganism identified in both groups.
- No significant difference in the overall count of microorganism colonies was observed between the water-only and soap-and-water bathing groups.
- Both bathing methods significantly reduced bacterial colony counts over time, with no significant difference between the groups.
Conclusions:
- Bathing preterm neonates with water only or with mild soap and water yields comparable outcomes regarding skin colonization.
- Both bathing strategies are effective in reducing the number of Gram-positive and Gram-negative bacterial colonies on preterm infant skin.
Objective:
To assess the effect of bathing with water only or with mild pH neutral soap and water on skin flora of preterm newborns.
Method:
Randomized blinded clinical trial that enrolled 73 preterm newborns whose gestational age was between 28 and 35 weeks and birth weight between 800 and 1800 g. At the age of 3 days the infants were randomly assigned to a group that was given daily baths either with water only, or with soap and water for 7 or more days. Antibiotic treatment delayed onset of the trial in some preterm infants. On the final day of bathing axillary bacterial cultures were obtained before and 30 minutes after bathing.
Results:
At the time of axillary culturing, the mean age of the newborns was 19 days. Coagulase-negative Staphylococcus was the most prevalent microorganism in both groups. No significant difference was found in the count of microorganism colonies between both groups. The comparison of the number of bacterial colonies between the time before and after bath, carried out by repeated-measures ANOVA showed a significant difference over time in the two groups, without a significant difference between the two groups.
Conclusions:
Bathing preterm newborns with water only or with soap and water produces similar effects on skin colonization of a preterm neonate. Both are effective to decrease the number of colonies of Gram-positive and Gram-negative bacteria.
Related Concept Videos
Development of the Oral Microbiota
Development of Human Microbiota
The Skin Microbiota
Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
Microbiota Modulation by Antibiotics
Anatomy of the Intestines
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the small...
