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[Apolipoproptein E-CI-CII gene cluster and its effect on serum lipid levels]

Chun-Hong Wang1, Xin Zhou, Qun Shi

  • 1School of Medicine, Wuhan University, Wuhan 430071, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research
|May 3, 2005
PubMed

Insights

Apolipoprotein (apo)E-CI-CII gene cluster frequencies in Chinese Hans were analyzed. ApoCII T1/2 genotype was linked to higher triglyceride levels, potentially explaining lower atherosclerosis rates in this population.

Area of Science:

  • Genetics
  • Biochemistry
  • Population Health

Background:

  • The apolipoprotein (apo)E-CI-CII gene cluster plays a role in lipid metabolism.
  • Understanding the genetic variations within this cluster is crucial for population-specific health assessments.

Purpose of the Study:

  • To determine the allele frequencies of the apoE-CI-CII gene cluster in the Han Chinese population.
  • To investigate the association between these gene polymorphisms and serum lipid levels.

Main Methods:

  • Genotyping of apoE, apoCI, and apoCII using PCR-based methods (multi-AMRS PCR, PCR-RFLP).
  • Serum lipid levels measured using an automatic chemistry analyzer.
  • Analysis of genotype distributions, allele frequencies, and linkage disequilibrium.

Main Results:

  • ApoE E3/3 (73.4%) was the most common genotype; rare genotypes (E2/2, E2/4, E4/4) were <1%.
  • ApoCI H1/1 (79.7%) and H2/2 (1.7%) frequencies were determined.
  • ApoCII allele frequencies were T1 (70.7%) and T2 (29.3%).
  • No association found between apoE or apoCI polymorphisms and plasma lipid levels.
  • The T1/2 genotype of ApoCII showed significantly higher triglyceride levels (P < 0.05) compared to T1/1.
  • No significant gender-based differences in genotype or allele frequencies were observed.

Conclusions:

  • The apoE-CI-CII gene cluster exhibits specific allele frequencies in the Han Chinese population.
  • ApoCII T1/2 genotype is associated with elevated triglyceride levels.
  • These findings may contribute to understanding the lower prevalence of atherosclerosis in China.
Abstract

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