Haptoglobin beta chain isoforms in the plasma and peritoneal fluid of women with endometriosis
Simone Ferrero1, David John Gillott, Valentino Remorgida
1Reproductive Physiology Laboratory, St. Bartholomew's School of Medicine and Dentistry, London, United Kingdom. simone.ferrero@fastwebnet.it
Objective:
To estimate the expression of haptoglobin (Hp) beta chain isoforms in the peritoneal fluid (PF) and plasma (PL) of women with and without endometriosis.
Design:
Cross-sectional study.
Setting:
University hospital.
Patient(S):
Seventy-two patients with endometriosis and 35 controls.
Intervention(S):
Peritoneal fluid and PL samples were subjected to two-dimensional gel electrophoresis, silver stained, digitally captured, and compared by semiquantitative computerized analysis.
Main Outcome Measure(S):
Expression of Hp beta chain isoforms.
Result(S):
No significant difference was observed in the frequency of expression of the Hp beta chain isoforms between the two groups. One beta chain isoform (HpbetaE; molecular weight, 38.40 +/- 0.94 kD; and isoelectric point, 5.63 +/- 0.17) had significantly higher expression in both the PF and PL of women with endometriosis than in the PF and PL of controls. No significant difference was observed in HpbetaE expression between women with mild (revised American Fertility Society [rAFS], stage I-II) and severe (rAFS, stage III-IV) endometriosis. In the control group, HpbetaE expression was correlated with the phase of the menstrual cycle.
Conclusion(S):
Women with endometriosis have higher PF and PL levels of HpbetaE. Further investigation should be aimed at producing antibodies against the HpbetaE-specific epitopes to determine whether its measurement may improve the clinical diagnosis of endometriosis.
