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Related Experiment Videos

C-CAM (Cell-CAM 105) is a calmodulin binding protein.

I Blikstad1, T Wikström, M Aurivillius

  • 1Department of Medical and Physiological Chemistry, University of Uppsala.

FEBS Letters
|May 4, 1992
PubMed
Summary
This summary is machine-generated.

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Cell-CAM 105, a cell adhesion molecule, specifically binds to calmodulin. This interaction occurs at the cytoplasmic domain of Cell-CAM 105 in a calcium-dependent manner.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell-CAM 105 (also known as C-CAM) is a transmembrane cell adhesion molecule.
  • It belongs to the immunoglobulin superfamily and mediates intercellular adhesion in rat hepatocytes.
  • C-CAM is found in various isoforms across epithelia, endothelia, and leukocytes.

Purpose of the Study:

  • To investigate the interaction between purified liver C-CAM and calmodulin.
  • To determine the binding site of calmodulin on C-CAM.
  • To identify C-CAM as a calmodulin-binding protein in liver membranes.

Main Methods:

  • Dot-blot assay using 125I-labeled C-CAM.
  • Gel overlay assay using 125I-labeled calmodulin.
  • Protease digestion to generate peptides for binding analysis.

Related Experiment Videos

  • Analysis of whole liver membranes.

    • Main Results:

    • Purified liver C-CAM specifically interacts with calmodulin.
    • Calmodulin binds to the cytoplasmic domain of C-CAM.
    • C-CAM is one of five major proteins in whole liver membranes that bind calmodulin in a calcium-dependent manner.

    Conclusions:

    • Cell-CAM 105 interacts directly with calmodulin.
    • The binding site is located within the cytoplasmic domain of Cell-CAM 105.
    • This interaction is calcium-dependent, suggesting a role in cellular signaling or regulation.