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Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen
Published on: January 26, 2024
Recombinant non-structural 1 (NS1) protein of dengue-2 virus interacts with human STAT3beta protein
John Jia-En Chua1, Raghavan Bhuvanakantham, Vincent Tak-Kwong Chow
1Programme in Infectious Diseases and Flavivirology Laboratory, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Kent Ridge, Singapore 117597, Singapore.
Abstract:
A combination of yeast two-hybrid library screening, co-immunoprecipitation and immunofluorescence microscopy demonstrated that dengue-2 virus non-structural 1 (NS1) protein can interact with an N-terminally truncated form of human STAT3beta (DeltaN40-STAT3beta) protein. The NS1 protein interacted with the activated STAT3beta protein in vesicle-like structures in the cell cytoplasm. In addition, transfection of dendritic cells with plasmid expressing NS1 protein also resulted in significant induction of tumor necrosis factor-alpha (TNFalpha) and interleukin-6 (IL-6). Since the STAT3beta protein is an acute-phase response factor, its interaction with NS1 protein may influence the pathological changes observed in dengue fever, dengue hemorrhagic fever and dengue shock syndrome.
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