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Updated: Aug 1, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Published on: September 14, 2013
[Involvement of non-clostridial anaerobic microorganisms in pathogenesis of chronic osteomyelitis]
Abstract:
The microbic pattern of osteomyelitic foci were analyzed in patients with chronic osteomyelitis on the AB Expression Analyzer by the 'BioMerieux" anaerobic cultivation and identification system (France). The osteomyelitis-focus microcenosis was shown to be of the mixed anaerobic-aerobic nature. Anaerobic microorganisms were isolated in 65.4% of examined patients and made 34% of the isolated bacterial cultures, which is indicative of an important role of anaerobes in the development and persistence of the purulent-inflammatory process in chronic osteomyelitis. Thus, it is feasible to prescribe the antibacterial drugs, which are active in respect to both aerobic and anaerobic bacteria.
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