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Updated: Aug 18, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
[Liquid chromatography frontal analysis of the protein binding of glimepiride]
Da-wei Zhou1, Huai-feng Wang, Fa-mei Li
1College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China. dawei1204@yahoo.com
Aim:
To study the protein binding of glimepiride.
Methods:
An HPLC-FA method is performed by using Pinkerton GFF II-S5-80 internal-surface reversed-phase silica support (150 mm x 4.6 mm ID, 5 microm) at pH 7.4 in a 67 mmol x L(-1) isotonic sodium phosphate buffer at 37 degree C. Other conditions included flow rate of 0.2 mL x min(-1), UV detection at wavelength 230 nm and injection volume 900 microL.
Results:
Nonlinear regression parameter estimation was used for the association constant measurement of glimepiride to both primary and secondary sites, which were 5.1 (micromol x L(-1)-1 and 1 for K1 and n1, and 0.017 (micromol x L(-1))-1 and 7 for K2 and n2, respectively.
Conclusion:
The method is shown to be suitable for investigation of protein binding of glimepiride.
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