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Susceptibility testing methods of antifungal agents
1College of Pharmacy, University of Iowa, Iowa City, USA.
Methods in Molecular Medicine
|May 13, 2005
Summary
Evaluating antifungal susceptibility is crucial. This summary covers methods for minimum inhibitory concentration and fungicidal testing, aiding in result interpretation for better antifungal therapy.
Area of Science:
- Mycology
- Clinical Microbiology
- Pharmacology
Background:
- Antifungal susceptibility testing (AFST) is essential for guiding antifungal drug selection.
- Current methods include standardized tests for minimum inhibitory concentrations (MICs) and various fungicidal assays.
- Interpreting AFST results requires understanding the principles behind different methodologies.
Purpose of the Study:
- To provide a comprehensive overview of current antifungal susceptibility testing methods.
- To explain the principles and applications of both MIC and fungicidal testing.
- To offer guidance on the utilization and evaluation of AFST data.
Main Methods:
- Standardized noncommercial and commercial assays for determining MICs.
- In vitro methods for fungicidal testing, including time-kill assays and minimum fungicidal concentration (MFC) determination.
- In vivo animal models for assessing antifungal efficacy.
Main Results:
- Detailed description of various standardized and non-standardized AFST techniques.
- Comparison of in vitro and in vivo approaches for antifungal evaluation.
- Guidelines for interpreting results from different susceptibility assays.
Conclusions:
- Effective utilization of AFST requires a thorough understanding of available methods.
- Accurate interpretation of MIC and fungicidal test results is key to optimizing antifungal treatment strategies.
- This chapter serves as a foundational resource for researchers and clinicians involved in antifungal therapy.