Neuronal, neurohormonal, and autocrine control of Xenopus melanotrope cell activity

Amphibian pituitary melanotropes are used to investigate principles of neuroendocrine translation of neural input into hormonal output. Here, the steps in this translation process are outlined for the melanotrope cell of Xenopus laevis, with attention to external stimuli, neurochemical messengers, receptor dynamics, second-messenger pathways, and control of the melanotrope secretory process. Emphasis is on the pathways that neurochemical messengers follow to reach the melanotrope. The inhibitory messengers, dopamine, gamma-aminobutyric acid, and neuropeptide Y, act on the cells by synaptic input from the suprachiasmatic nucleus, whereas the locus coeruleus and raphe nucleus synaptically stimulate the cells via noradrenaline and serotonin, respectively. Autoexcitatory actions are exerted by acetylcholine, brain-derived neurotrophic factor (BDNF), and the calcium-sensing receptor. At least six messengers released from the pituitary neural lobe stimulate melanotropes in a neurohormonal way: corticotropin-releasing hormone, thyrotropin-releasing hormone, BDNF, urocortin, mesotocin, and vasotocin. They all are produced by the magnocellular nucleus and coexist in various combinations in two types of neurohemal axon terminal. Most of the relevant receptors of the melanotropes have been elucidated. Apparently, the neural lobe has a dominant role in activating melanotrope secretory activity. The intracellular mechanisms translating the various inputs into cellular activities like biosynthesis and secretion constitute the adenylyl cyclase-cAMP pathway and Ca(2+) in the form of periodic changes of the intracellular Ca(2+) concentration, known as Ca(2+) oscillations. It is proposed that the pattern of these oscillations encodes specific regulatory information and that it is set by first messengers that control, for example, via G proteins and cAMP-related events, specific ion channel-mediated events in the membrane of the melanotrope cell.