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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
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Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain

Tasawan Singhsilarak1, Wattana Leowattana, Sornchai Looareesuwan

  • 1Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmtss@mahidol.ac.th

The American Journal of Tropical Medicine and Hygiene
|May 14, 2005
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...