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Updated: Aug 18, 2026

Inducible and Reversible Dominant-negative (DN) Protein Inhibition
Published on: January 7, 2019
delta-Opioid agonist induced regulation of E2F1 DNA binding activity in NG108-15 cells
Zvetanka S Tencheva1, Maria D Praskova, Adriana A Velichkova
1Institute of Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str. Blok 23, 1113 Sofia, Bulgaria. Tencheva@mail.bol.bg
Abstract:
Activation of opioid receptors have been implicated in the modulation of cell proliferation and the E2F family of transcription factors may play a role in opioid inhibition of DNA synthesis. Gel shift assays and Western blotting of nuclear extracts from NG108-15 cells revealed increased E2F1 DNA binding activity and higher levels of E2F1 following activation of delta-opioid receptors. It is suggested that DADLE-induced regulation of E2F DNA binding activity involves ERKs.
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