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Updated: Aug 18, 2026

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
Published on: April 20, 2012
A new dihydroxysterol from the marine phytoplankton Diacronema sp
Amélia P Rauter1, Manuela M Filipe, Carla Prata
1Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, Ed. C8, 5 piso, 1749-016 Lisboa, Portugal. aprauter@fc.ul.pt
Abstract:
Diacronema sp. was cultured and its sterols were separated by column chromatography on silica gel. The new sterol 24-ethyl-4alpha-methyl-cholestane-3,20-diol (1) was characterised by NMR and MS spectrometry, as well as (22E)-24-ethyl-4alpha-methyl-5alpha-cholest-22-en-3beta-ol (2) and beta-sitosterol, the major components of the sterol fractions. Neither the biosynthetic origin of the new dihydroxysterol nor its role in the biochemistry of Diacronema is known.
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