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Lanthanide-based luminescent assays for ligand-receptor interactions
Heather L Handl1, Robert J Gillies
1Arizona Cancer Center, P.O. Box 245024, 1515 N. Campbell, Tucson, AZ 85724, USA.
Life Sciences
|May 17, 2005
Summary
Lanthanide labels offer a sensitive and efficient alternative for monitoring receptor-ligand interactions in drug discovery. Time-resolved fluorescence (TRF) assays using lanthanides improve throughput and reduce assay time and cost.
Area of Science:
- Biochemistry
- Pharmacology
- Analytical Chemistry
Background:
- Receptor-ligand interaction evaluation is crucial for drug discovery.
- Current methods like radiolabels are low-throughput, while fluorescent methods have limitations.
- A need exists for more sensitive and widely applicable labels.
Purpose of the Study:
- To introduce lanthanides as novel labels for receptor-ligand interaction assays.
- To highlight the advantages of lanthanide labels over traditional methods.
- To review recent assays employing lanthanides for interaction studies.
Main Methods:
- Utilizing lanthanides as labels coupled to ligands.
- Employing time-resolved fluorescence (TRF) detection methods.
- Detecting attomole levels of labels in multiwell plate samples.
Main Results:
- Lanthanide labels provide strong luminescent signals detectable by TRF.
- TRF assays with lanthanides offer high sensitivity, detecting less than one attomole of europium.
- Lanthanide incorporation makes assays more affordable, faster, and automatable.
Conclusions:
- Lanthanides are attractive alternatives to traditional labels for receptor-ligand interaction studies.
- TRF assays with lanthanides enhance sensitivity, throughput, and efficiency.
- This approach facilitates drug discovery and development by improving assay performance.