An automated tube-type blood viscometer: validation studies

Area of Science:

• Biomedical Engineering
• Hematology

Background:

• Previous rheometers presented technical complexity, limiting blood viscosity data availability.
• Automated viscometers are needed for efficient and accessible blood viscosity analysis.

Purpose of the Study:

• To validate the accuracy and reproducibility of the automated tube-type viscometer, Rheolog.
• To assess the impact of storage conditions on blood viscosity measurements using the Rheolog.

Main Methods:

• Validation of the Rheolog using normal human blood against cone-plate and Couette viscometers.
• Evaluation of blood viscosity data at varying hematocrit levels and shear rates (1-1500 s(-1)).
• Assessment of storage time and temperature effects on blood viscosity measurements.

Main Results:

• Rheolog measurements showed high reproducibility (CV < 5%) and were independent of hematocrit and shear rate.
• Rheolog blood viscosity data closely correlated with established viscometer methods across different hematocrit levels.
• Minimal impact of storage at room temperature (up to 8 hours) and 4°C (up to 4 days); significant changes observed after 3 hours at 37°C.

Conclusions:

• The Rheolog offers a rapid, accurate, and reproducible method for determining blood viscosity.
• This automated viscometer is suitable for both basic scientific research and clinical studies.
• Optimal storage conditions are crucial for maintaining the integrity of blood viscosity measurements.