Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional fluorescence lifetime tomography.

Anuradha Godavarty1, Eva M Sevick-Muraca, Margaret J Eppstein

  • 1Department of Computer Science, University of Vermont, Burlington, Vermont 05405, USA. godavart@fiu.edu

Medical Physics
|May 18, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Turep: Detecting cross-cancer tumor-reactive T cells in single-cell and spatial transcriptomics data.

bioRxiv : the preprint server for biology·2026
Same author

Telomerase Knockout in Myeloid Cells Predisposes Mice to Foam Cell Formation, Dyslipidemia, Lung Fibrosis, and Cardiac Dysfunction.

Aging cell·2026
Same author

A Novel Spatial Correlation Analysis of Peripheral Hemodynamics in a Murine Model of Vascular Calcification Using NIRS Imaging.

Annals of biomedical engineering·2026
Same author

Lymphatic pumping failure in the arm precedes dermal backflow and breast cancer-related lymphedema.

Breast cancer research : BCR·2026
Same author

Self-Attention Diffusion Models for Zero-Shot Biomedical Image Segmentation: Unlocking New Frontiers in Medical Imaging.

Bioengineering (Basel, Switzerland)·2025
Same author

Mathematical modeling and analysis for tissue curvature correction in near-infrared spectroscopy imaging.

Journal of biomedical optics·2025
Same journal

A novel optical respiratory gating system with a hybrid phase-amplitude algorithm for spot-scanning proton therapy.

Medical physics·2026
Same journal

Gamma Knife treatment planning using knowledge-based reinforcement learning.

Medical physics·2026
Same journal

Development and characterization of a novel, small animal external beam irradiator using a clinical high dose rate brachytherapy source.

Medical physics·2026
Same journal

Deep learning-based dose prediction for MR-guided prostate SIB: Supporting rapid feasibility assessment and adaptive editing margin selection.

Medical physics·2026
Same journal

Surface-guided analysis of breast shape changes during postoperative radiotherapy by using a functional map framework.

Medical physics·2026
Same journal

Monte Carlo assessment of a treatment planning system for intraoperative radiotherapy in the presence of tissue heterogeneities.

Medical physics·2026
See all related articles

This study establishes the feasibility of 3D fluorescence lifetime tomography for early cancer diagnostics. It demonstrates the ability to image fluorescent contrast agents deep within tissue using a novel Kalman filter reconstruction algorithm.

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Fluorescence Tomography

Background:

  • Near-infrared fluorescence tomography with targeted agents aids early cancer detection.
  • Fluorescence Lifetime Imaging Microscopy (FLIM) is common in microscopy but limited to 2D in medical imaging.

Purpose of the Study:

  • To establish the feasibility of 3D fluorescence lifetime tomography for medical imaging.
  • To develop and validate a system for deep-tissue fluorescence lifetime imaging.

Main Methods:

  • Utilized a gain-modulated intensified CCD and modulated laser diode imaging system.
  • Employed two fluorescent contrast agents with distinct lifetimes (Indocyanine green, Diethylthiatricarbocyanine iodide).
  • Applied a two-stage approximate extended Kalman filter for image reconstruction.

Related Experiment Videos

Main Results:

  • Successfully demonstrated 3D fluorescence lifetime tomography on clinically relevant phantom volumes.
  • Achieved imaging at depths of 1.4-2 cm with high target-to-background ratios (70:1, 100:1).
  • Obtained 3D images of lifetime and absorption using a Bayesian tomography algorithm.

Conclusions:

  • 3D fluorescence lifetime tomography is feasible for medical applications, particularly for early cancer diagnostics.
  • The developed system and algorithm enable deep-tissue imaging of fluorescent contrast agents based on their lifetime properties.