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Updated: Aug 18, 2026
An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
[Concentration and determination of strychnine alkaloid in biological fluids]
Jing Zhang1, Lang-chong He, Qiang Fu
1School of medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Objective:
To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers.
Methods:
A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids.
Results:
the limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.9966 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit.
Conclusion:
The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.
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