Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conformationally constrained PNA analogues: structural evolution toward DNA/RNA binding selectivity.

Vaijayanti A Kumar1, Krishna N Ganesh

  • 1Division of Organic Synthesis, National Chemical Laboratory, Pune 411008, India. vakumar@dalton.ncl.res.in

Accounts of Chemical Research
|May 18, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Celebrating a Decade of <i>ACS Omega</i>: Reflections from the Editorial Roundtable.

ACS omega·2025
Same author

Supramolecular multiplexes from collagen mimetic peptide-PNA(GGG)<sub>3</sub> conjugates and C-rich DNA: pH-induced reversible switching from triplex-duplex to triplex-<i>i</i>-motif.

Biomaterials science·2024
Same author

Supramolecular polyplexes from Janus peptide nucleic acids (bm-PNA-G5): self-assembled bm-PNA G-quadruplex and its tetraduplex with DNA.

Organic & biomolecular chemistry·2024
Same author

Dynamics of terminal fraying-peeling and hydrogen bonds dictates the sequential <i>vs</i>. cooperative melting pathways of nanoscale DNA and PNA triplexes.

Nanoscale·2024
Same author

<i>ACS Omega</i>'s Progress Report: Growing with Trust and Diversity.

ACS omega·2023
Same author

Unlike RNA-TBA (rTBA), iso-rTBA, the 2'-5'-linked RNA-thrombin-binding aptamer, is a functional equivalent of TBA.

Chemical communications (Cambridge, England)·2023
Same journal

Innate Immunity of Framework Nucleic Acids.

Accounts of chemical research·2026
Same journal

High-Performance CH-Series Non-Fullerene Acceptors for Organic Photovoltaics.

Accounts of chemical research·2026
Same journal

Design Principles for Negative Thermal Expansion in Two-Dimensional Materials.

Accounts of chemical research·2026
Same journal

Main Group Redox Catalysis: New Frontiers with Germanium and Tin.

Accounts of chemical research·2026
Same journal

Taming Irreversibility in sp<sup>2</sup>-Carbon-Conjugated COFs from Polycrystalline Powders to Single Crystals and Thin Films.

Accounts of chemical research·2026
Same journal

Electroactive Imidazolium Ionic Liquids in Organic Synthesis.

Accounts of chemical research·2026
See all related articles

Researchers engineered locked nucleic acid (LNA) analogues for peptide nucleic acid (PNA) to specifically bind DNA or RNA targets. This advancement improves PNA

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medicinal Chemistry

Background:

  • Peptide nucleic acids (PNAs) are DNA mimics with therapeutic and diagnostic potential.
  • Current PNA analogues lack sufficient discrimination between DNA and RNA targets for in vivo applications.
  • Engineering PNA for selective binding is crucial for advancing their clinical utility.

Purpose of the Study:

  • To design and synthesize conformationally constrained PNA analogues for enhanced DNA/RNA discrimination.
  • To investigate the impact of structural preorganization on PNA binding affinity and specificity.
  • To develop PNA locked nucleic acid (LNA) hybrids for targeted nucleic acid recognition.

Main Methods:

  • Design of conformationally restricted PNA backbone structures.

Related Experiment Videos

  • Chemical synthesis of novel PNA analogues, including locked PNA (LNA-PNA).
  • Evaluation of hybridization specificity and binding affinity using biophysical techniques.

    • Main Results:

    • Conformationally constrained PNA analogues exhibit improved binding selectivity for DNA or RNA.
    • Locked PNA (LNA-PNA) designs demonstrate increased binding strength without compromising specificity.
    • The study provides a rational design strategy for developing highly specific PNA-based nucleic acid binders.

    Conclusions:

    • Structurally preorganized PNA analogues offer a viable strategy for selective DNA/RNA targeting.
    • Locked PNA (LNA-PNA) hybrids represent a promising class of molecules for diagnostic and therapeutic applications.
    • Further development of these PNA analogues could lead to novel nucleic acid-based therapies.