[A study of forensic individual identification used STR locus with silver staining and multiplex PCR methods]

Amplification of short tandem repeat(STR) loci has become a useful tool for human identification applications. To improve throughput and efficiency for the forensic materials and gain foure and six STR locis multiplex methods with silver staining, CSF1PO,TPOX,THO1 and vWA(referred to as multiplex A), D18S51, D7S820, D13S317, D5S818, D3S1358 and Amelogenin(referred to as multiplex B) have been evaluated for use in a rape case. The products of multiplex amplication were separated in a denaturing polyacrylamide gel and analyzed with silver staining. Two multiplex amplications used in this case could provide a power of discrimination of approximately 2.43 x 10(-19). Silver staining was shown to be a validation methods for analysing the products of four and six multiplex amplications.