Related Experiment Videos
Evi-1 expression in Xenopus.
Paul E Mead1, Evan Parganas, Shiro Ohtsuka
1Department of Pathology, St Jude Children's Research Hospital, Memphis, TN 38105, USA. paul.mead@stjude.org
Gene Expression Patterns : GEP
|May 21, 2005
Summary
The Evi-1 gene, a transcription factor linked to myeloid leukemia, is cloned and studied in Xenopus. Its expression is observed during oogenesis and embryonic development, particularly in pronephric tissue and neural crest derivatives.
Area of Science:
- Developmental biology
- Molecular genetics
- Cancer research
Background:
- The Evi-1 gene was identified in murine myeloid leukemia.
- Evi-1 is a zinc finger transcription factor implicated in myeloid neoplasia.
- Chromosomal rearrangements involving the Evi-1 locus are associated with human myeloid leukemia and myelodysplastic syndromes.
Purpose of the Study:
- To clone and characterize the Xenopus Evi-1 (xEvi-1) gene.
- To investigate the developmental expression pattern of xEvi-1.
Main Methods:
- Gene cloning
- In situ hybridization
- Analysis of gene expression during oogenesis and embryonic development.
Main Results:
- xEvi-1 was successfully cloned.
- xEvi-1 exhibits dynamic expression during oogenesis and early embryonic development.
- Expression was detected in pronephric tissue, brain, and neural crest derivatives.
Conclusions:
- xEvi-1 plays a role in Xenopus development.
- The findings provide insights into the conserved function of Evi-1 in vertebrate development and potentially in oncogenesis.