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Related Experiment Videos

Agonist-induced calcium response in single human platelets assayed in a microfluidic device.

Louie Tran1, Javier Farinas, Lily Ruslim-Litrus

  • 1Caliper Technologies, Mountain View, CA 94043, USA.

Analytical Biochemistry
|May 24, 2005
PubMed
Summary

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Researchers developed a new method for isolating and loading platelets with fluorophores. This technique enables functional testing of platelet responses to drugs using microfluidics for drug discovery.

Area of Science:

  • Biomedical Engineering
  • Hematology
  • Drug Discovery

Background:

  • Platelet-specific targets are crucial for drug discovery.
  • Existing methods for studying platelet function can be limited.
  • High-throughput screening platforms are needed for efficient drug development.

Purpose of the Study:

  • To develop a method for isolating and loading human platelets with fluorophores.
  • To establish a microfluidics-based screening platform for platelet signaling.
  • To demonstrate proof-of-principle for drug discovery targeting platelets.

Main Methods:

  • Developed a platelet isolation and fluorophore-loading protocol.
  • Optimized ratiometric determination of agonist-induced calcium flux.
  • Utilized a microfluidic device with specific cell density and flow rates.

Related Experiment Videos

  • Tested platelet response to adenosine diphosphate and thrombin receptor-activating peptide (TRAP).

    • Main Results:

    • Achieved functionally responsive, fluorophore-loaded platelets.
    • Detected agonist-induced calcium flux in a microfluidic platform.
    • Optimized microfluidic parameters for event resolution (5-20 platelets/unit time).
    • Observed dose-dependent platelet responses to agonists.

    Conclusions:

    • The developed method enables functional platelet studies.
    • Microfluidics offers a viable platform for high-throughput platelet drug screening.
    • This approach facilitates drug discovery targeting platelet-specific pathways.