Related Experiment Video
Updated: Aug 17, 2026
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
Published on: April 23, 2015
[Establishment of COL1A1-EGFP stably transfected ROS17/2.8 cell lines]
Zhong-quan Dai1, Ying-hui Li, Bai Ding
1Institute of Space Medico-Engineering, Beijing, China.
Objective:
To obtain ROS17/2.8 cell lines which were stably expressing EGFP reporter gene drived by COL1A1 promoter.
Method:
A 3.6 Kb COL1A1 promoter from rat was cloned into pMD-18-T vector by PCR. This amplified promoter vector was digested to get several different length fragments which were then fused with EGFP reporter gene to construct eukaryotic expression vectors. ROS17/2.8 cell was stably transfected with these vectors by LipofectAMINE(TM) and selected by G418.
Result:
The COL1A1-EGFP stably transfected cell lines were established.
Conclusion:
The cell lines will be useful for studying the effects of microgravity on the activity of COL1A1 promoter and expression of gene related with bone form.
