Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altering genes in animals by gene targeting.

B H Koller1, O Smithies

  • 1Department of Medicine, University of North Carolina, Chapel Hill 27599-7020.

Annual Review of Immunology
|January 1, 1992
PubMed
Summary

Scientists can now create genetically modified mice by altering embryonic stem (ES) cells. This technique enables the study of gene function in vivo, particularly for the immune system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuronal NLRP1 inflammasome activation of Caspase-1 coordinately regulates inflammatory interleukin-1-beta production and axonal degeneration-associated Caspase-6 activation.

Cell death and differentiation·2015
Same author

Unique populations of lung mast cells are required for antigen-mediated bronchoconstriction.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2010
Same author

Assessment of NMDA receptor activation in vivo by Fos induction after challenge with the direct NMDA agonist (tetrazol-5-yl)glycine: effects of clozapine and haloperidol.

Journal of neural transmission (Vienna, Austria : 1996)·2007
Same author

Ocular inflammatory responses in the EP2 and EP4 receptor knockout mice.

Ocular immunology and inflammation·2006
Same author

Characterization of Brca1 deficient mice.

Breast disease·2005
Same author

Genetic contributions to generalized arousal of brain and behavior.

Proceedings of the National Academy of Sciences of the United States of America·2003

Area of Science:

  • Genetics
  • Developmental Biology
  • Immunology

Background:

  • Genetic engineering allows for precise modifications within an organism's genome.
  • Embryonic stem cells (ES cells) are crucial for generating genetically modified animals.
  • Homologous recombination is a key molecular mechanism for targeted DNA modification.

Purpose of the Study:

  • To describe a method for generating genetically altered mice.
  • To highlight the utility of this technique for studying gene function in vivo.
  • To emphasize the application in understanding immune system development and function.

Main Methods:

  • Targeted genomic alteration in mouse embryonic stem (ES) cells via homologous recombination.
  • Injection of modified ES cells into recipient blastocysts.
  • Development of chimeric mice from injected blastocysts.
  • Germline transmission of the engineered genetic alteration.

Main Results:

  • Successful generation of mice with planned genomic alterations.
  • Demonstration of ES cell contribution to the germline in chimeric mice.
  • Establishment of a method for heritable genetic modification in mice.

Conclusions:

  • The described technique provides a powerful tool for genetic manipulation in mice.
  • This method facilitates in vivo studies of gene function, particularly in immunology.
  • Genetically engineered mice are valuable models for biological research.

Related Experiment Videos