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Novel bacterial phylotypes in endodontic infections
J F Siqueira1, I N Rôças, C D Cunha
1Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil. siqueira@estacio.br
Journal of Dental Research
|May 26, 2005
Summary
Molecular analysis revealed uncultivated Spirochaetes and Deferribacteres bacteria in root canals. These findings suggest these bacteria may be pathogens contributing to periradicular diseases.
Area of Science:
- Microbiology
- Endodontics
- Molecular Biology
Background:
- Molecular studies suggest Spirochaetes and Deferribacteres phyla contain oral pathogens.
- The presence of these specific bacterial phyla in endodontic infections requires further investigation.
Purpose of the Study:
- To investigate the occurrence and diversity of Spirochaetes and Deferribacteres in endodontic infections.
- To identify potential pathogens involved in the etiology of periradicular diseases.
Main Methods:
- Nested PCR-DGGE (Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis) approach was used.
- Samples from infected root canals were analyzed for target bacterial groups, including Atopobium species.
- Sequencing of amplified DNA fragments identified bacterial species and phylotypes.
Main Results:
- Eight out of ten infected root canal samples showed bands representing the target bacterial groups.
- DGGE profiles indicated a mean of 6.5 intense and faint bands per sample.
- Sequences from intense bands were similar to Flexistipes species (Deferribacteres), uncharacterized spirochetes, and Atopobium species.
Conclusions:
- Uncultivated Spirochaetes and Deferribacteres phylotypes are frequently detected in the endodontic microbiota.
- These bacterial groups may act as potential pathogens contributing to the development of periradicular diseases.