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Updated: Aug 17, 2026
11:12
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Insertion of unmarked DNA sequences in multiple loci of the Bacillus subtilis 168 genome: an efficient selection
Rie Uotsu-Tomita1, Shinya Kaneko, Kenji Tsuge
1Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan.
Bioscience, Biotechnology, and Biochemistry
|May 26, 2005
Abstract:
The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.
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