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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Utilization of methyloleate in production of microbial lipase
Jacqueline Destain1, Patrick Fickers, Frédéric Weekers
1Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés, 2, B-5030 Gembloux, Belgium. destain.j@fsagx.ac.be
Abstract:
In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase. Our results demonstrate that methyloleate, a cheap hydrophobic compound, could efficiently substitute olive oil as the inducer and carbon source for lipase production. A new process of lipase production was developed yielding a twofold increase in the level of production compared with the levels in previous reports.
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