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Related Experiment Videos

[Clinical experience with a modified method of human embryo vitrification].

R Hredzák1, A Ostró, V Zdilová

  • 1Centrum asistovanej reprodukcie, II, gynekologicko-pôrodnicka klinika LF UPJS a FN L. Pasteura, Kosice, Slovenská republika.

Ceska Gynekologie
|May 28, 2005
PubMed
Summary

This study presents a simplified, cost-effective vitrification method for human embryos created via in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). The modified technique demonstrated reliable embryo survival and development, leading to successful pregnancies.

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Area of Science:

  • Reproductive Medicine
  • Embryology
  • Cryobiology

Context:

  • Supernumerary human embryos from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles require efficient cryopreservation.
  • Traditional slow-rate freezing methods can be complex and time-consuming.

Purpose:

  • To evaluate the clinical efficacy of a modified vitrification technique for human embryos.
  • To assess embryo survival, development, and pregnancy rates using this simplified method.

Summary:

  • A modified vitrification protocol using increasing cryoprotectant concentrations (40% ethylene glycol, 1 M sucrose) and a 100 microl pipetting tip was applied to 215 human embryos.
  • Post-thaw embryo survival was 69.8%, with 48% capable of further development.
  • Clinical pregnancy rates were 19.0% per thawing cycle and 27.6% per transfer, resulting in 8 live births.

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Impact:

  • The modified vitrification procedure offers a reliable, simpler, shorter, and more economical alternative to slow-rate freezing for human embryos.
  • This method can improve the efficiency and accessibility of assisted reproductive technologies.