Related Experiment Videos
[Clinical experience with a modified method of human embryo vitrification]
R Hredzák1, A Ostró, V Zdilová
1Centrum asistovanej reprodukcie, II, gynekologicko-pôrodnicka klinika LF UPJS a FN L. Pasteura, Kosice, Slovenská republika.
Ceska Gynekologie
|May 28, 2005
Summary
This study presents a simplified, cost-effective vitrification method for human embryos created via in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). The modified technique demonstrated reliable embryo survival and development, leading to successful pregnancies.
Area of Science:
- Reproductive Medicine
- Embryology
- Cryobiology
Context:
- Supernumerary human embryos from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles require efficient cryopreservation.
- Traditional slow-rate freezing methods can be complex and time-consuming.
Purpose:
- To evaluate the clinical efficacy of a modified vitrification technique for human embryos.
- To assess embryo survival, development, and pregnancy rates using this simplified method.
Summary:
- A modified vitrification protocol using increasing cryoprotectant concentrations (40% ethylene glycol, 1 M sucrose) and a 100 microl pipetting tip was applied to 215 human embryos.
- Post-thaw embryo survival was 69.8%, with 48% capable of further development.
- Clinical pregnancy rates were 19.0% per thawing cycle and 27.6% per transfer, resulting in 8 live births.
Impact:
- The modified vitrification procedure offers a reliable, simpler, shorter, and more economical alternative to slow-rate freezing for human embryos.
- This method can improve the efficiency and accessibility of assisted reproductive technologies.