Glycine toxicokinetics: vitreous fluid concentration and visual impairment

We report the results of kinetic experiments in sheep. Following systemic loading with 45 g glycine, plasma, cerebral spinal fluid (CSF), and vitreous fluid from the vitreous body of the eye were assayed for glycine concentrations. Mean baseline plasma, CSF, and vitreous glycine concentrations were 730, 46, and 191 mumol/L respectively. One hour after loading, values were 21292, 1103, and 1150 mumol/L respectively. During the succeeding 7 h plasma and CSF concentrations fell in parallel but remained above normal. In contrast the vitreous concentrations remained high and did not change significantly during the 7 h post-infusion period. Retinal glycine receptors may be particularly vulnerable to neurotoxic effects of glycine because of these pharmacokinetic properties.