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The ischemic rat heart releases S100B
Guilherme S Mazzini1, Débora V Schaf, Alvaro R Oliveira
1Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul. Rua Ramiro Barcelos, 2600, anexo.CEP 90035-003. Porto Alegre, RS, Brazil.
Life Sciences
|June 1, 2005
Summary
The injured heart releases S100B, a protein previously used to detect brain injuries. This study confirms the ischemic heart as an extra-cerebral source of S100B, impacting its use as a specific neurological marker.
Area of Science:
- Biochemistry
- Cardiology
- Neurology
Background:
- S100B is an astrocytic protein used as a biomarker for cerebral injuries.
- Extra-cerebral sources may influence serum S100B levels.
- The injured myocardium has been reported to express S100B.
Purpose of the Study:
- To investigate whether the isolated heart releases S100B.
- To determine if myocardial ischemia and reperfusion lead to S100B release.
Main Methods:
- Isolated rat hearts were perfused using the Langendorff technique.
- Hearts were subjected to 20 minutes of ischemia followed by 30 minutes of reperfusion (ischemic group) or 50 minutes of perfusion (control group).
- Perfusion fluid was analyzed for S100B and cardiac troponin T levels at various time points.
Main Results:
- S100B and cardiac troponin T levels significantly increased in the ischemic group after reperfusion.
- Median S100B levels rose from <0.02 µg/L to 0.38 µg/L.
- Median troponin T levels increased from 0.31 µg/L to 2.84 µg/L.
Conclusions:
- The ischemic heart releases S100B.
- This finding identifies the heart as an extra-cerebral source of S100B.
- The results suggest that serum S100B levels may be influenced by cardiac injury.