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Messenger RNA expression of periostin and Twist transiently decrease by occlusal hypofunction in mouse periodontal
Elaine Afanador1, Masahiko Yokozeki, Yasuo Oba
1Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.
Archives of Oral Biology
|June 1, 2005
Summary
Occlusal force influences periostin and Twist gene expression in the periodontal ligament (PDL). Reduced force led to decreased expression, suggesting an adaptive response in PDL cells.
Area of Science:
- Biochemistry
- Cell Biology
- Dental Research
Background:
- Periostin (a cell adhesion protein) and Twist (a transcription factor) are crucial in periodontal ligament (PDL) homeostasis.
- Occlusal force is recognized as a key factor in maintaining PDL structure and function.
Purpose of the Study:
- To investigate the impact of occlusal hypofunction on periostin and Twist mRNA expression in mouse periodontal tissue.
- To elucidate the role of mechanical stress in regulating these key genes within the PDL.
Main Methods:
- Unilateral maxillary tooth extraction was performed in mice to induce occlusal hypofunction.
- Real-time PCR and in situ hybridization were used to quantify periostin and Twist mRNA levels at various time points post-extraction.
Main Results:
- Periostin and Twist mRNA expression significantly decreased at 24 hours post-extraction, indicating sensitivity to reduced mechanical load.
- Expression levels began to recover by 72 and 168 hours, suggesting an adaptive mechanism.
- In situ hybridization confirmed a reduction in periostin and Twist mRNA-expressing cells at 24 hours, with recovery by 168 hours.
Conclusions:
- Occlusal force plays a significant role in regulating periostin and Twist gene expression in the PDL.
- The observed changes in gene expression during hypofunction represent an adaptive response of PDL cells to altered mechanical environments.