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Determination of methyltestosterone using flow injection with chemiluminescence detection
Zenghong Xie1, Xiaoqing Ouyang, Liangqia Guo
1Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, People's Republic of China.
Abstract:
Methyltestosterone (MT) enhanced the chemiluminescence of potassium permanganate-sodium thiosulphate system in sulphuric acid medium, and this was used as the basis of a novel flow-injection chemiluminescence method for the determination of methyltesterone. Under optimum conditions, the linear response of MT was in the range 3.0 x 10(-8)-8.0 x 10(-6) g/mL, and the detection limit for determination of MT was 1.0 x 10(-8) g/mL. The method was applied to the analysis of fish with recovery in the range 88.0-113.8% with an average RSD of 1.66% (n = 8).
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