Related Experiment Video
Updated: Aug 17, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Flow injection analysis of riboflavin with chemiluminescence detection using a N-halo compounds-luminol system
A Safavi1, M A Karimi, M R Hormozi Nezhad
1Department of Chemistry, Faculty of Sciences, Shiraz University, Shiraz 71454, Iran. safavi@chem.susc.ac.ir
Abstract:
A chemiluminescent method for the determination of riboflavin is described. The method is based on the chemiluminescence (CL) generated during the oxidation of luminol by N-bromosuccinimide (NBS) and N-chlorosuccinimide (NCS) in alkaline medium. It was found that riboflavin could greatly enhance this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method for the determination of riboflavin is proposed in this paper. The detection limits were 7.5 ng/mL and 3.5 ng/mL of riboflavin for the NBS- and NCS-luminol CL systems, respectively. The relative CL intensity was linear, with riboflavin concentration in the range 19-600 ng/mL and 600-2000 ng/mL for the NBS-luminol CL system, and 12-200 ng/mL and 200-2000 ng/mL for the NCS-luminol CL system. The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official method and demonstrated good accuracy and precision.
More Related Videos
08:23Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
08:25Characterizing Riboglow Probes In Vitro as the Basis for Fluorescence Lifetime Imaging In Live Mammalian Cells and Three-Dimensional Cellular Models
Published on: August 4, 2026
Related Concept Videos
Photoluminescence: Applications
Flame Photometry: Lab
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Flame Photometry: Overview