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Related Experiment Videos

Hepatic dysoxia commences during O2 supply dependence.

R Schlichtig1, H A Klions, D J Kramer

  • 1Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh, Pennsylvania.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|April 1, 1992
PubMed
Summary

The study investigated if reduced liver oxygen consumption during low oxygen delivery indicates tissue dysoxia. Findings suggest that decreased oxygen consumption is indeed a sign of dysoxia, not an adaptive response.

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Area of Science:

  • Physiology
  • Hepatology
  • Metabolism

Background:

  • Hepatic oxygen consumption (VO2) is typically independent of oxygen delivery (DO2) until a critical point (DO2c).
  • The reason for decreased VO2 below DO2c, whether adaptive or due to dysoxia, is unclear.

Purpose of the Study:

  • To test the hypothesis that reduced hepatic VO2 during O2 supply dependence signifies tissue dysoxia.
  • To evaluate hepatic mitochondrial NAD redox state as an indicator of dysoxia.

Main Methods:

  • Progressive hemorrhage was induced in pentobarbital-anesthetized dogs to create O2 supply independence and dependence.
  • Hepatic mitochondrial NAD redox state was estimated using the beta-hydroxybutyrate-to-acetoacetate ratio (beta OHB/AcAc) in hepatic venous blood.
  • Lactate extraction ratio was also measured.

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Main Results:

  • The critical DO2 (DO2c) was 5.02 +/- 1.64 ml.100 g-1.min-1.
  • The beta OHB/AcAc ratio remained constant until a DO2 of 3.03 +/- 1.08 ml.100 g-1.min-1, after which it increased linearly, indicating altered mitochondrial redox state.
  • Peak liver lactate extraction was 15.2 +/- 14.1% at a DO2 of 5.48 +/- 2.54 ml.100 g-1.min-1.

Conclusions:

  • The observed increase in the beta OHB/AcAc ratio supports the hypothesis that reduced hepatic VO2 during O2 supply dependence is a manifestation of tissue dysoxia.
  • This suggests that the liver's ability to maintain oxygen consumption is compromised under conditions of severe oxygen deprivation.
  • The findings highlight the importance of monitoring indicators of cellular oxygenation beyond simple delivery rates.