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Updated: Aug 17, 2026
Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Simultaneous determination of nine fluoroquinolones in egg white and egg yolk by liquid chromatography with
Zhenling Zeng1, Aiguo Dong, Guixiang Yang
1Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642 Guangdong, China.
Abstract:
A liquid chromatographic (LC) method with fluorescence detection was developed for determination of nine fluoroquinolones (FQs) in egg white and yolk. Egg white samples were deproteinized with acidified ethanol (egg yolk samples with acetonitrile and acidified ethanol), followed by defatting with hexane once (white) or twice (yolk), and extracting FQs into acetonitrile. After acetonitrile was evaporated, the residue was dissolved in mobile phase, and FQs were detected in LC with a fluorescence detector. Recoveries for nine FQs from white and yolk were 74.7-85.6%, 79.1-91.2%, respectively, with excellent relative standard deviations. The limits of quantification were 5-20 ngg(-1).
