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Apelin cells in the rat stomach.
Etsuko Susaki1, Guiyun Wang, Gan Cao
1Department of Histology and Cell Biology, School of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Regulatory Peptides
|June 2, 2005
Summary
Apelin peptide is produced by multiple cell types in the rat stomach, including mucous neck, parietal, chief, and enteroendocrine cells. These findings highlight apelin
Area of Science:
- Gastroenterology
- Endocrinology
- Cell Biology
Background:
- Apelin is an endogenous peptide and the ligand for the APJ receptor.
- Apelin expression is found in various tissues, including the central nervous system, heart, lung, mammary gland, and gastrointestinal tract.
Purpose of the Study:
- To identify specific cell types within the rat stomach that produce apelin peptide.
- To investigate the localization of apelin-producing cells in the gastric mucosa.
Main Methods:
- Immunohistochemistry (IHC) was employed to detect apelin peptide in rat stomach tissue.
- Gastric epithelial cells were analyzed for apelin expression and co-localization with markers like chromogranin A (CGA).
Main Results:
- Apelin-positive cells were predominantly found in the neck and upper base regions of gastric glands within the mucosal epithelium.
- Apelin was detected in mucous neck cells, parietal cells, chief cells, and enteroendocrine cells (marked by CGA).
- Apelin was notably absent in the muscle layer of the stomach.
Conclusions:
- Apelin is expressed in both gastric exocrine (mucous neck, parietal, chief cells) and endocrine (enteroendocrine cells) lineages.
- These findings support previous data indicating measurable levels of apelin in gut lumen and systemic circulation.