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Lactosucrose production by various microorganisms harboring levansucrase activity
Nyun-Ho Park1, Ha-Jong Choi, Deok-Kun Oh
1Department of Bioscience and Biotechnology, Sejong University, Seoul, 143-747, Korea.
Biotechnology Letters
|June 2, 2005
Summary
Bacillus subtilis efficiently produces the artificial sweetener lactosucrose using levansucrase. Optimal conditions yielded 181 g/L lactosucrose from lactose and sucrose in 10 hours.
Area of Science:
- Biotechnology
- Enzymology
- Food Science
Background:
- Levansucrase enzymes catalyze the synthesis of fructooligosaccharides.
- Microbial production of functional sweeteners is an area of growing interest.
- Lactosucrose is an artificial sweetener with potential applications in food and pharmaceuticals.
Purpose of the Study:
- To investigate the production of lactosucrose by microorganisms with levansucrase activity.
- To identify the most effective microbial producer of lactosucrose.
- To determine the optimal conditions for lactosucrose synthesis.
Main Methods:
- Screening of various microorganisms for levansucrase activity.
- Enzymatic synthesis of lactosucrose using lactose as an acceptor and sucrose as a fructosyl donor.
- Optimization of reaction parameters including pH, temperature, and substrate concentrations.
Main Results:
- Bacillus subtilis was identified as the most effective bacterial producer of lactosucrose.
- Optimal production conditions were determined to be pH 6.0 and 55°C.
- Under optimal conditions, 181 g/L of lactosucrose was produced from 225 g/L lactose and 225 g/L sucrose in 10 hours.
Conclusions:
- Bacillus subtilis is a highly efficient microbial cell factory for lactosucrose production.
- Optimized enzymatic synthesis offers a viable route for industrial-scale lactosucrose manufacturing.
- Further research can explore strain improvement and process scale-up for enhanced lactosucrose yields.