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Updated: Aug 17, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
[Charactierization of a new antibacterial polypeptide isolated from human cervical mucas]
Ming Li1, Xiao-ling Pan, Li-li Wang
1Research Unit of Infection & Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
Objective:
To identify a new antibactrial polypeptide HCP-1 isolated from human cervical mucus.
Methods:
HCP-1 was isolated and purified from human cervical mucus, and N-terminal sequence of HCP-1 was determined. A degenerate primer was designed according to CodeHop methods, and an "anchor-oliga-dT primer" was used for the synthesis of cDNA. Using the degenerate primer and anchor-primer, cDNAs were amplified by PCR. The PCR products were cloned, sequenced, and analyzed by biological software.
Results:
N-terminat sequence of HCP-1 was PKRKAEGDAK. The full length of HCP-1 cDNA was isolated and of which the sequence was the same as HMG-17. OMIGA software analysis indicated that this molecule contained an alpha-helix region.
Conclusion:
The new antibacterial polypeptide isolated from human cervical mucus is HMG-17. It may play a role in the human cervical mucus and the alpha-helix domain may be its antibacterial activity region.
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