Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatocyte viability and protein expression within hydrogel microstructures.

Laura J Itle1, Won-Gun Koh, Michael V Pishko

  • 1Department of Chemical Engineering, The Huck Institute for the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802-4420, USA.

Biotechnology Progress
|June 4, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gelatin methacryloyl hydrogel with post-injection magnetic alignment: Structural modulation and protein-cell interactions.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Flexible Dielectric Acoustic Resonator Patch for Tissue Regeneration.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Low-dose radiation generated ROS-activatable doxorubicin prodrug loaded liposome nanoparticles for triple-negative breast cancer treatment.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Hydroxyapatite-Mediated Mechanical Modulation of GelMA Hydrogels Influences Osteogenic Differentiation of 3D Spheroids.

Gels (Basel, Switzerland)·2026
Same author

Esophagus extracellular matrix with microenvironmental complexity for esophageal organoids.

Bioactive materials·2026
Same author

Multilayer surface coating for enhanced anti-inflammation, anti-restenosis, and re-endothelialization in advanced biodegradable vascular stents.

Materials today. Bio·2025

Poly(ethylene) glycol (PEG) hydrogels support mammalian hepatocytes, but higher PEG concentrations reduce overall protein production. The RGD peptide significantly enhances albumin production while decreasing fibronectin in PEG hydrogels.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Biotechnology

Background:

  • Poly(ethylene) glycol (PEG) hydrogels are utilized for cell encapsulation in drug screening and biosensing.
  • Understanding the impact of hydrogel composition on cellular function is crucial for optimizing these applications.

Purpose of the Study:

  • To investigate how PEG hydrogel composition influences protein production by mammalian hepatocytes.
  • To assess the effects of the RGD peptide on specific protein secretion within PEG hydrogels.

Main Methods:

  • Mammalian hepatocytes were cultured within PEG hydrogels for 7 days.
  • Cell viability, total protein, albumin, and fibronectin production were quantified.
  • The influence of varying PEG concentrations and the presence of the RGD peptide was analyzed.

Related Experiment Videos

Main Results:

  • PEG hydrogel composition did not affect hepatocyte viability.
  • Increased PEG concentration led to a significant decrease in total protein production.
  • Hepatocytes in PEG hydrogels produced more protein than those in traditional monolayer cultures.
  • The RGD peptide addition dramatically increased albumin production and decreased fibronectin production in 10% PEG hydrogels.

Conclusions:

  • PEG hydrogel composition impacts cellular protein synthesis, with higher concentrations reducing output.
  • The RGD peptide can selectively modulate specific protein secretion (albumin and fibronectin) in PEG hydrogels.
  • PEG hydrogels, particularly with RGD modification, offer a promising platform for enhanced protein production compared to monolayer cultures.