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Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Published on: January 7, 2013
Correlation between loss of PTEN expression and PKB/AKT phosphorylation in hepatocellular carcinoma
Zhifang Yang1, Jilin Yi, Xingrui Li
1Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Abstract:
The clinical significance of phosphatase and tensin homolog deleted on chromosome ten (PTEN) protein expression and the correlation between the expression of PTEN and phosphorylation of protein kinase B (PKB/AKT) in human hepatocellular carcinoma (HCC) were investigated. The expression of PTEN and phospho-AKT was detected by SP immunohistochemical technique and Western blotting in 35 cases of HCC, 15 cases of liver cirrhosis and 8 cases of normal tissues. The correlation between the expression of PTEN and PKB/AKT in HCC was analyzed. The results showed that the positive expression of PTEN in HCC (62.9%, 0.085 +/- 0.021) was significantly lower than that in liver cirrhosis and normal tissues (P < 0. 01). The expression level of PTEN was related to the differentiation degree of HCC and the status of metastasis (P < 0.05). Western blotting revealed a significant inverse correlation between PTEN and phospho-AKT (r = -0.818, P < 0.01). These results demonstrated that down-regulation or loss of PTEN, which may not be able to effectively inhibit the hyper-phosphorylation of PKB/AKT, might play an important role in tumorigenesis and progression of HCC.
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