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Updated: Aug 17, 2026
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
[Molecular genetic studies on ganglioglioma]
1Department of Pathology, Renji Hospital Affiliated to Shanghai Second Medical University, Shanghai 200127, China. niyin@yahoo.com
Objective:
To study the genetic alterations of ganglioglioma through the entire genome, and to investigate the pathogenesis of this neoplasm.
Methods:
Comparative genomic hybridization was used to provide an overview of genetic abnormalities in gangliogliomas.
Results:
Five cases of gangliogliomas, including 3 males and 2 females, were studied genetically. Loss of genetic materials on the short arm of chromosome 9(9p) was a common genetic alteration found in 3 of 5 cases. Overrepresentation of chromosome 7 was another recurrent chromosomal imbalance, which was further confirmed by fluorescence in situ hybridization. Immunohistochemical analysis was performed on epidermal growth factor receptor (EGFR), which was located on 7p11-p13. All five cases revealed no abnormal expression of EGFR. On the other hand, genetic imbalances were also involved in multiple chromosomes including 2q33-q34, 8q12-q22, 14q21-qter, 15q26-qter and Y.
Conclusion:
Loss of genetic materials on chromosome 9p and gain on chromosome 7 may be associated with the pathogenesis of this neoplasm.
