Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Linkage mapping bovine EST-based SNP.

Warren M Snelling1, Eduardo Casas, Roger T Stone

  • 1US Meat Animal Research Center, Agricultural Research Service, US Department of Agriculture, Clay Center, Nebraska 68933-0166, USA. snelling@email.marc.usda.gov

BMC Genomics
|June 10, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pangenome-based structural variant imputation enables large-scale genotype-phenotype studies in dairy cattle.

Nature communications·2026
Same author

Leukotoxin enhances nasopharyngeal colonization and shedding of Mannheimia haemolytica in bovine herpesvirus 1 infected calves.

Research in veterinary science·2026
Same author

A chromosome level reference genome for the pecan weevil, Curculio caryae.

Scientific data·2026
Same author

Detection of <i>Mycoplasma bovirhinis</i> and bovine coronavirus in an outbreak of bovine respiratory disease in nursing beef calves.

Frontiers in microbiomes·2026
Same author

Identification of cis-regulatory elements provides insights into tissue-specific gene regulation in the sheep genome.

Nature communications·2026
Same author

Impact of Methylparaben on Cumulus Cell DNA Integrity and Porcine Oocyte Developmental Competence In Vitro.

Journal of applied toxicology : JAT·2026
Same journal

Different genomic footprint of small insertion-deletion and structural variants determines the genetic divergence of indica and japonica rice.

BMC genomics·2026
Same journal

From nurse bee to queen egg: RNA-seq analysis of Apis mellifera eggs shows dietary protein-dependent gene regulation.

BMC genomics·2026
Same journal

A genome-wide association study to identify the genetic loci underlying carbapenem resistance in Acinetobacter baumannii.

BMC genomics·2026
Same journal

Comparative transcriptome analysis to reveal key drought stress-responsive genes in sorghum (Sorghum bicolor (L.) Moench).

BMC genomics·2026
Same journal

Tissue identity is the dominant determinant of cross-species transferability of a porcine developmental programme.

BMC genomics·2026
Same journal

Characterization of mitochondrial genomes from three medicinal species of rutaceae and comparative analysis within the family: insights into evolution.

BMC genomics·2026
See all related articles

This study developed 918 new single nucleotide polymorphism (SNP) markers for the bovine genome, significantly enhancing the linkage map. This improved map aids in fine-mapping quantitative trait loci (QTL) and identifying genes for economically important traits in cattle.

Area of Science:

  • Genomics
  • Animal Genetics
  • Bioinformatics

Background:

  • Existing bovine genome linkage maps rely heavily on anonymous microsatellite markers.
  • Current maps are suitable for broad quantitative trait loci (QTL) mapping but lack the resolution for fine-mapping.
  • There is a need for gene-associated markers to identify specific genes influencing QTL.

Purpose of the Study:

  • To develop and integrate new single nucleotide polymorphism (SNP) markers into the bovine linkage map.
  • To increase marker density and provide connections to gene-rich physical maps.
  • To facilitate fine-mapping of QTL and identification of candidate genes in cattle.

Main Methods:

  • Developed 918 SNP markers using bovine expressed sequence tag (EST) and bacterial artificial chromosome (BAC) sequence data.

Related Experiment Videos

  • Genotyped SNPs in a reference population using DNA from sires, progeny, and mates.
  • Determined chromosome assignments and positions using two-point and multipoint linkage analyses.

    • Main Results:

    • Successfully mapped 861 SNPs and established positions for 735 SNPs.
    • The updated bovine linkage map contains 4585 markers (3612 microsatellites, 913 SNPs) spanning 3058 cM with a mean marker separation of 1.2 cM.
    • Identified homologous human sequences for 1159 markers, including 582 newly developed SNPs.

    Conclusions:

    • The addition of EST- and BAC-based SNPs enhances marker density and links to gene-rich physical maps.
    • The improved map is a valuable resource for fine-mapping QTL and identifying positional candidate genes in cattle.
    • This map will aid in understanding the genetic basis of economically important traits in beef and dairy cattle.