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Updated: Aug 17, 2026

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice
Published on: July 13, 2017
Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS
G Liu1, I H Martins, J A Chiorini
1Program in Gene Therapy, University of Iowa, Iowa City, IA 52242, USA.
Abstract:
The subventricular zone (SVZ) is one of the neurogenic niches in the adult mammalian brain. The SVZ is of interest for studies on neurogenesis and stem cell therapy. Here, we report specific transduction of ependyma and/or astrocytes by recombinant adeno-associated virus type 4 (AAV4) viral vectors. AAV4 vectors encoding beta-galactosidase or eGFP were injected into the lateral ventricles of neonatal and adult C57BL/6 mouse brains. In addition, SVZ injections were conducted on adult mice. AAV4 vectors show a characteristic transduction of the ependyma independent of delivery route. However, AAV4 virus injected into the SVZ targeted GFAP positive astrocytes forming the glial tube in the SVZ and rostral migratory stream (RMS). Our results introduce AAV4 as a new tool by which to manipulate glial cells in the RMS.
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