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Related Experiment Videos

Spectral-domain phase microscopy.

Michael A Choma1, Audrey K Ellerbee, Changhuei Yang

  • 1Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA. mac32@duke.edu

Optics Letters
|June 11, 2005
PubMed
Summary

Spectral-domain phase microscopy (SDPM) detects nanometer-scale cellular motions using broadband interferometry. This technique, an extension of optical coherence tomography, was validated on beating heart cells, showing high sensitivity for cellular dynamics.

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Area of Science:

  • Biomedical Optics
  • Cellular Biophysics
  • Optical Coherence Tomography

Background:

  • Broadband interferometry offers depth-resolved phase information for detecting cellular motion.
  • Coherence gating is crucial for isolating phase signals in biological samples.

Purpose of the Study:

  • Introduce spectral-domain phase microscopy (SDPM) as a novel phase-sensitive technique.
  • Extend spectral-domain optical coherence tomography (SD-OCT) for cellular motion detection.
  • Quantify the sensitivity and displacement limits of SDPM.

Main Methods:

  • Developed SDPM as a functional extension of SD-OCT.
  • Demonstrated and calibrated the nanometer-scale motion detection sensitivity.
  • Derived the shot-noise limit for displacement sensitivity.

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  • Applied SDPM to study dynamics in chick embryo cardiomyocytes.

    • Main Results:

    • SDPM successfully detects nanometer-scale motions in living cells.
    • The technique's sensitivity was experimentally verified and calibrated.
    • A theoretical shot-noise limit for displacement sensitivity was established.
    • Cellular dynamics in spontaneously beating cardiomyocytes were measured.

    Conclusions:

    • SDPM is a highly sensitive technique for measuring nanometer-scale cellular dynamics.
    • The method provides a valuable tool for studying cell biophysics and function.
    • SDPM advances the application of optical coherence tomography in live-cell imaging.